Journal: International Journal of Molecular Sciences

Article Title: Estrogen α and β Receptor Expression in the Various Regions of Resected Glioblastoma Multiforme Tumors and in an In Vitro Model

doi: 10.3390/ijms25074130

Figure Lengend Snippet: Expression of ERα gene ( A ) and ERα protein ( B ) in U87 cells cultured under different conditions. Data are representative of each group cultured in control, nutrient-deficient, hypoxic, and necrotic conditions. Statistical analysis was performed using t -test *, p < 0.005.

Article Snippet: Cell culture of the U87 line (obtained from the European Collection of Authenticated Cell Cultures (ECACC)) was performed under standard conditions of 37 °C, 95% humidity, and 5% CO 2 , according to the manufacturer’s instructions.

Techniques: Expressing, Cell Culture, Control

Journal: International Journal of Molecular Sciences

Article Title: Estrogen α and β Receptor Expression in the Various Regions of Resected Glioblastoma Multiforme Tumors and in an In Vitro Model

doi: 10.3390/ijms25074130

Figure Lengend Snippet: Representative images taken with the FV1000 confocal microscope system (Olympus, Hamburg, Germany) show ERα protein expression in U87 cells cultured under specific conditions: control ( A ), nutrient deficiency ( B ), hypoxia ( C ), and necrotic conditions ( D ). FITC (AR) and DAPI (nuclear) markers were used. Microphotographs were taken at ×20 magnification ( A , B , D ) and ×40 magnification ( C ); scale bar 30 µm.

Article Snippet: Cell culture of the U87 line (obtained from the European Collection of Authenticated Cell Cultures (ECACC)) was performed under standard conditions of 37 °C, 95% humidity, and 5% CO 2 , according to the manufacturer’s instructions.

Techniques: Microscopy, Expressing, Cell Culture, Control

Journal: International Journal of Molecular Sciences

Article Title: Estrogen α and β Receptor Expression in the Various Regions of Resected Glioblastoma Multiforme Tumors and in an In Vitro Model

doi: 10.3390/ijms25074130

Figure Lengend Snippet: Expression of the ERβ gene ( A ) and ERβ protein ( B ) in U87 cells cultured under different conditions. Data are representative of each group cultured in control, nutrient-deficient, hypoxic, and necrotic conditions. Statistical analysis was performed using a t -test * p < 0.05.

Article Snippet: Cell culture of the U87 line (obtained from the European Collection of Authenticated Cell Cultures (ECACC)) was performed under standard conditions of 37 °C, 95% humidity, and 5% CO 2 , according to the manufacturer’s instructions.

Techniques: Expressing, Cell Culture, Control

Journal: International Journal of Molecular Sciences

Article Title: Estrogen α and β Receptor Expression in the Various Regions of Resected Glioblastoma Multiforme Tumors and in an In Vitro Model

doi: 10.3390/ijms25074130

Figure Lengend Snippet: Representative images taken with the FV1000 confocal microscope system (Olympus, Hamburg, Germany) show ERβ protein expression in U87 cells cultured under specific conditions: control ( A ), nutrient deficiency ( B ), hypoxia ( C ), and necrotic conditions ( D ). FITC (AR) and DAPI (nuclear) markers were used. Microphotographs were taken at ×20 magnification ( A , B , D ) and ×40 magnification ( C ); scale bar 30 µm.

Article Snippet: Cell culture of the U87 line (obtained from the European Collection of Authenticated Cell Cultures (ECACC)) was performed under standard conditions of 37 °C, 95% humidity, and 5% CO 2 , according to the manufacturer’s instructions.

Techniques: Microscopy, Expressing, Cell Culture, Control

Journal: Nature Communications

Article Title: Late-stage (radio)fluorination of alkyl phosphonates via electrophilic activation

doi: 10.1038/s41467-024-54208-y

Figure Lengend Snippet: a Late-stage radiofluorination of PET tracers and 18 F-synthons. RCC TLC determined by radio-TLC ( n = 3); RCY = isolated 18 F-product activity amount/starting amount of radioactivity (decay corrected). cLog P values were predicted using ALOGPS 2.1 ( http://www.vcclab.org/lab/alogps ). Blue arrows pointing upward indicate an increase in cLog P /Log D 7.4 compared to the parent compound, while downward arrows indicate a decrease. The small pink ball after cLog P /Log D 7.4 represents the target of the parent compound. b Preparation α v β 3 integrin receptor developer [ 18 F]BFPA-E[c(RGDyK)] 2 . c MicroPET images of [ 18 F]BFPA-E[c(RGDyK)] 2 in U87MG xenograft mice at 30 min after tail vein injection. 200 μg of E[c(RGDyK)] 2 was used to block the tumor uptake of [ 18 F]BFPA-E[c(RGDyK)] 2 . The white circle is the tumor area. d Preparation of PET tracer [ 18 F]BFPA-Flurpiridaz targeting MC I. e MicroPET/CT images of healthy mice [ 18 F]BFPA-Flurpiridaz 60 min after caudal vein injection. f Molecular Docking. Flurpiridaz and BFPA-Flurpiridaz to MC I (PDB: 7ZM8). Cyan: residues composing the substrate-binding cavity; yellow: residues forming hydrogen bonds; yellow dashed lines: locations of hydrogen bond.

Article Snippet: The glioma cell line U87MG was obtained from the China Center for Type Culture Collection of the Chinese Academy of Sciences.

Techniques: Isolation, Activity Assay, Radioactivity, Injection, Blocking Assay, Binding Assay

Journal: Scientific Reports

Article Title: HSV1 microRNAs in glioblastoma development: an in silico study

doi: 10.1038/s41598-023-45249-2

Figure Lengend Snippet: The volcano plots of the included samples from the GSE158284 and GSE13276 datasets and the common genes between DEGs and targets of hsv1-miR-H6-3p and hsv1-miR-H1-5p. ( A ) The differentially expressed genes (DEGs) of included samples from the GSE158284 dataset with the cut-off of adjusted P-value ˂ 0.05 and |log2FC| ≥ 1.3. ( B ) The DEGs of the included samples from the GSE158284 dataset adjusted P-value ˂ 0.05 and |log2FC| ≥ 1.3. (C) The common genes between DEGs and targets of hsv1-miR-H6-3p and hsv1-miR-H1-5p. ( C ) The common genes between targets of hsv1-miR-H6-3p and the downregulated genes in GBM tissues. ( D ) The common genes between targets of hsv1-miR-H1-5p and the upregulated genes in GBM tissues.

Article Snippet: The Human Protein Atlas ( https://www.proteinatlas.org/ ) was used to identify the protein localization of DEGs in human GBM cell lines .

Techniques:

Journal: Cancers

Article Title: Targeting Glutamine Addiction in Gliomas

doi: 10.3390/cancers12020310

Figure Lengend Snippet: Therapeutic approaches targeting glutamine addiction in gliomas.

Article Snippet: , SAS , D54MG, U87MG, U251MG, STTG1 human GBM cell lines; patient-derived GBM cells; mouse intracranial xenograft (D54MG cells) , decreased cell proliferation and migration; decreased tumor growth and invasion , [ , ] .

Techniques: Inhibition, In Vitro, shRNA, Migration, Over Expression, Activity Assay, In Vivo

Journal: Toxins

Article Title: Cytotoxicity Effect of Quinoin, Type 1 Ribosome-Inactivating Protein from Quinoa Seeds, on Glioblastoma Cells

doi: 10.3390/toxins13100684

Figure Lengend Snippet: IC 50 values estimation. IC 50 values of U87Mg cells and two primary glioblastoma cell lines NULU and ZAR after 24, 48, and 72 h of incubation with quinoin using concentrations of 0.01, 0.1, 1.0, 2.5, and 5.0 μM. The control was assumed as part of the dose–response curve, considering it as a very low concentration (10 −11 µM). Data were processed using GraphPad Prism and data are reported as Mean ± SD.

Article Snippet: The U87Mg human GBM cell lines were obtained from the Sigma Aldrich Collection (LGC Promochem, Teddington, UK).

Techniques: Incubation, Control, Concentration Assay

Journal: Toxins

Article Title: Cytotoxicity Effect of Quinoin, Type 1 Ribosome-Inactivating Protein from Quinoa Seeds, on Glioblastoma Cells

doi: 10.3390/toxins13100684

Figure Lengend Snippet: Growth curve and MTT assay of the U87Mg glioblastoma continuous cell line and primary cell lines. ( A ) On the left, the graphs of the growth curves of the continuous glioblastoma cell line U87Mg and of two primary cell lines obtained from the patient’s biopsy (NULU and ZAR) are shown. Quinoin was administered at various doses of 2.5 and 5.0 nM daily, at various time intervals (1, 2, 3 days). ( B ) On the right, the graphs of the cell viability assessed by MTT assay. U87Mg and patient-derived glioblastoma cell lines NULU and ZAR treated daily with quinoin 2.5 and 5.0 nM, at various time intervals (1, 2, 3 days). Data shown are representative of three separate experiments and values are presented as Mean ± SEM. Statistical analysis was performed by one-way ANOVA. According to GraphPad Prism, * p -value 0.01 to 0.05 (significant), ** p -value 0.001 to 0.01 (very significant), *** p -value 0.0001 to 0.001 (extremely significant), **** p -value < 0.0001 (extremely significant).

Article Snippet: The U87Mg human GBM cell lines were obtained from the Sigma Aldrich Collection (LGC Promochem, Teddington, UK).

Techniques: MTT Assay, Derivative Assay

Journal: Toxins

Article Title: Cytotoxicity Effect of Quinoin, Type 1 Ribosome-Inactivating Protein from Quinoa Seeds, on Glioblastoma Cells

doi: 10.3390/toxins13100684

Figure Lengend Snippet: Morphological change of quinoin-treated U87Mg. The glioblastoma continuous cell line was exposed to 0.01, 0.1, 1.0, 2.5, and 5.0 μM quinoin for 72 h. Cells were imaged with an Evos FL microscope at 20× magnification.

Article Snippet: The U87Mg human GBM cell lines were obtained from the Sigma Aldrich Collection (LGC Promochem, Teddington, UK).

Techniques: Microscopy

Journal: Scientific Reports

Article Title: Highly efficient radiosensitization of human glioblastoma and lung cancer cells by a G-quadruplex DNA binding compound

doi: 10.1038/srep16255

Figure Lengend Snippet: Results of 53BP1 immunofluorescence analysis of GBM (SF763, SF767) and NSCLC (A549, H1299) cells pre-treated by Pt-ctpy during 24 hours and irradiated with 2 Gy. Cells were fixed 0.5 h ( A ) or 24 h ( B ) after irradiation. ( A ) Representative images of SF763 cell nuclei fixed 0.5 h following irradiation stained with 4′,6-diamidino-2-phenylindole (DAPI, blue) and 53BP1 (green) exposed to Pt-ctpy and/or irradiation. The increase of 53BP1 foci number per cell was significant in GBM or NSCLC cells exposed to 0.2 μM Pt-ctpy and radiation compared with non-treated (NT) cells and cells treated with radiation alone (H-test). ( B ) Representative images of SF763 cell nuclei fixed 24 h after irradiation stained with 4′,6-diamidino-2-phenylindole (DAPI, blue) and 53BP1 (green) after exposure to Pt-ctpy and/or irradiation. The number of residual DSB 24 h after irradiation was significantly increased after combined treatment (H-Test).

Article Snippet: Human GBM cell lines SF763 and SF767 were kindly provided by Dr Moyal (UMR 1037 INSERM, Toulouse, France).

Techniques: Immunofluorescence, Irradiation, Staining