Journal: International Journal of Molecular Sciences

Article Title: Estrogen α and β Receptor Expression in the Various Regions of Resected Glioblastoma Multiforme Tumors and in an In Vitro Model

doi: 10.3390/ijms25074130

Figure Lengend Snippet: Expression of ERα gene ( A ) and ERα protein ( B ) in U87 cells cultured under different conditions. Data are representative of each group cultured in control, nutrient-deficient, hypoxic, and necrotic conditions. Statistical analysis was performed using t -test *, p < 0.005.

Article Snippet: Cell culture of the U87 line (obtained from the European Collection of Authenticated Cell Cultures (ECACC)) was performed under standard conditions of 37 °C, 95% humidity, and 5% CO 2 , according to the manufacturer’s instructions.

Techniques: Expressing, Cell Culture, Control

Journal: International Journal of Molecular Sciences

Article Title: Estrogen α and β Receptor Expression in the Various Regions of Resected Glioblastoma Multiforme Tumors and in an In Vitro Model

doi: 10.3390/ijms25074130

Figure Lengend Snippet: Representative images taken with the FV1000 confocal microscope system (Olympus, Hamburg, Germany) show ERα protein expression in U87 cells cultured under specific conditions: control ( A ), nutrient deficiency ( B ), hypoxia ( C ), and necrotic conditions ( D ). FITC (AR) and DAPI (nuclear) markers were used. Microphotographs were taken at ×20 magnification ( A , B , D ) and ×40 magnification ( C ); scale bar 30 µm.

Article Snippet: Cell culture of the U87 line (obtained from the European Collection of Authenticated Cell Cultures (ECACC)) was performed under standard conditions of 37 °C, 95% humidity, and 5% CO 2 , according to the manufacturer’s instructions.

Techniques: Microscopy, Expressing, Cell Culture, Control

Journal: International Journal of Molecular Sciences

Article Title: Estrogen α and β Receptor Expression in the Various Regions of Resected Glioblastoma Multiforme Tumors and in an In Vitro Model

doi: 10.3390/ijms25074130

Figure Lengend Snippet: Expression of the ERβ gene ( A ) and ERβ protein ( B ) in U87 cells cultured under different conditions. Data are representative of each group cultured in control, nutrient-deficient, hypoxic, and necrotic conditions. Statistical analysis was performed using a t -test * p < 0.05.

Article Snippet: Cell culture of the U87 line (obtained from the European Collection of Authenticated Cell Cultures (ECACC)) was performed under standard conditions of 37 °C, 95% humidity, and 5% CO 2 , according to the manufacturer’s instructions.

Techniques: Expressing, Cell Culture, Control

Journal: International Journal of Molecular Sciences

Article Title: Estrogen α and β Receptor Expression in the Various Regions of Resected Glioblastoma Multiforme Tumors and in an In Vitro Model

doi: 10.3390/ijms25074130

Figure Lengend Snippet: Representative images taken with the FV1000 confocal microscope system (Olympus, Hamburg, Germany) show ERβ protein expression in U87 cells cultured under specific conditions: control ( A ), nutrient deficiency ( B ), hypoxia ( C ), and necrotic conditions ( D ). FITC (AR) and DAPI (nuclear) markers were used. Microphotographs were taken at ×20 magnification ( A , B , D ) and ×40 magnification ( C ); scale bar 30 µm.

Article Snippet: Cell culture of the U87 line (obtained from the European Collection of Authenticated Cell Cultures (ECACC)) was performed under standard conditions of 37 °C, 95% humidity, and 5% CO 2 , according to the manufacturer’s instructions.

Techniques: Microscopy, Expressing, Cell Culture, Control

Journal: Toxins

Article Title: Cytotoxicity Effect of Quinoin, Type 1 Ribosome-Inactivating Protein from Quinoa Seeds, on Glioblastoma Cells

doi: 10.3390/toxins13100684

Figure Lengend Snippet: IC 50 values estimation. IC 50 values of U87Mg cells and two primary glioblastoma cell lines NULU and ZAR after 24, 48, and 72 h of incubation with quinoin using concentrations of 0.01, 0.1, 1.0, 2.5, and 5.0 μM. The control was assumed as part of the dose–response curve, considering it as a very low concentration (10 −11 µM). Data were processed using GraphPad Prism and data are reported as Mean ± SD.

Article Snippet: The U87Mg human GBM cell lines were obtained from the Sigma Aldrich Collection (LGC Promochem, Teddington, UK).

Techniques: Incubation, Control, Concentration Assay

Journal: Toxins

Article Title: Cytotoxicity Effect of Quinoin, Type 1 Ribosome-Inactivating Protein from Quinoa Seeds, on Glioblastoma Cells

doi: 10.3390/toxins13100684

Figure Lengend Snippet: Growth curve and MTT assay of the U87Mg glioblastoma continuous cell line and primary cell lines. ( A ) On the left, the graphs of the growth curves of the continuous glioblastoma cell line U87Mg and of two primary cell lines obtained from the patient’s biopsy (NULU and ZAR) are shown. Quinoin was administered at various doses of 2.5 and 5.0 nM daily, at various time intervals (1, 2, 3 days). ( B ) On the right, the graphs of the cell viability assessed by MTT assay. U87Mg and patient-derived glioblastoma cell lines NULU and ZAR treated daily with quinoin 2.5 and 5.0 nM, at various time intervals (1, 2, 3 days). Data shown are representative of three separate experiments and values are presented as Mean ± SEM. Statistical analysis was performed by one-way ANOVA. According to GraphPad Prism, * p -value 0.01 to 0.05 (significant), ** p -value 0.001 to 0.01 (very significant), *** p -value 0.0001 to 0.001 (extremely significant), **** p -value < 0.0001 (extremely significant).

Article Snippet: The U87Mg human GBM cell lines were obtained from the Sigma Aldrich Collection (LGC Promochem, Teddington, UK).

Techniques: MTT Assay, Derivative Assay

Journal: Toxins

Article Title: Cytotoxicity Effect of Quinoin, Type 1 Ribosome-Inactivating Protein from Quinoa Seeds, on Glioblastoma Cells

doi: 10.3390/toxins13100684

Figure Lengend Snippet: Morphological change of quinoin-treated U87Mg. The glioblastoma continuous cell line was exposed to 0.01, 0.1, 1.0, 2.5, and 5.0 μM quinoin for 72 h. Cells were imaged with an Evos FL microscope at 20× magnification.

Article Snippet: The U87Mg human GBM cell lines were obtained from the Sigma Aldrich Collection (LGC Promochem, Teddington, UK).

Techniques: Microscopy